Coding

Part:BBa_K2029003:Design

Designed by: Niklas Schmacke, Max Schelski   Group: iGEM16_UBonn_HBRS   (2016-10-14)


XynB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part codes for the Clostridium Cellulovorans xylanase xynB. It has sucessfully been expressed and secreted in B. subtilis strain WB800 using the plasmid pWB980 (KanR) (ref 1)


Source

Clostridium Cellulovorans

References

1 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1785254/ Arai T, Matsuoka S, Cho H-Y, et al. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proceedings of the National Academy of Sciences of the United States of America. 2007;104(5):1456-1460. doi:10.1073/pnas.0610740104.